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Objective To investigate the chemical constituents of the root of Wrightia pubescens.Methods Compounds were isolated by the combined use of colum chromatography,preparative HPLC and recrystallization,and their structures were identified by their physicochemical and spectroscopic data.Cytotoxic activities of the compounds were evaluated using MTT method in vitro.Re?sults Twelve compounds 1-12 were isolated from the ethyl acetate soluble part from 75% ethanol extract of the root of W.pubescens, and identified as scopoletin(1),coumarin(2),cleomiscosin B(3),mollugin(4),4-hydroxybenzoic acid(5),vanillic acid(6), vanillin(7),4-hydroxymethyl-5-hydroxy-2H-pyran-2-one(8),β-sitosterol(9),β-daucosterol(10),ursonic acid(11),and me?dioresinol(12). Compound 4 showed cytotoxic activity against HepG2 cells in vitro with IC50of 8.0 mg/L. The IC50of compound 11 against MCF-7 and HepG2 cells was 14.7 and 18.2 mg/L,respectively.Conclusion Compounds 1-5,8 and 12 were isolated from the genus Wrightia for the first time. Compounds 6 and 10 were isolated from the title plant for the first time. Compounds 4 and 11 showed cytotoxic activities against tumor cells in vitro.
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Objective To study the chemical constituents of the male anthotaxy of Populus tomentosa Carr.Methods The compounds were isolated and purified by ODS,silica gel,Sephadex LH-20 column chromatography and recrystallization.Their struc?tures were identified based on the physiochemical properties and spectroscopic data. The antioxidant capacities of some compounds were assayed by a rapid ABTS method.The antitumor activity was tested by the MTT assay.Results Fourteen compounds were isolat?ed from the ethyl acetate part of 70% ethanol extract of the male anthotaxy of P.tomentosa Carr.,and they were identified as pinocem?brin(1),dillenetin(2),chrysoeriol(3),naringenin(4),isosakuranetin(5),apigenin(6),kaempferol(7),apigenin-7-O-β-D(-6″-p-coumaroyl)-glucoside(8),tremuloidine(9),catechol(10),3,3′,4,4′-tetrahydroxybiphenyl(11),coumaric acid(12),ursolic acid (13),and betulonic acid(14),respectively. The results of the ABTS assay showed that 2,6 and 7 could scavenge ABTS+free radicals with the total antioxidant capacity of 0.61,0.14,0.46 respectively.The screening results of antitumor activity in vitro showed that the half inhibitory concentration(IC50)of compounds 1,4 and 5 for adenocarcinomic human alveolar basal epithelial cells A549 was 26.04 mg/L,43.45 mg/L and 24.01 mg/L,for human hepatocellular liver carcinoma cells HepG2 were 13.50 μg/ml,23.80 μg/ml and 9.13 μg/ml,and for human colon carcinoma cells HCT116 was 36.11 mg/L,48.95 mg/L and 32.25 mg/L.Conclusion Compounds 2,3,11,13 and 14 were isolated from the Populus genus for the first time,2-5,10,11 and 13-15 were isolated from the plant for the first time.Compounds 2,6 and 7 displayed the antioxidant activity to a certain extent.Compouds 1,4 and 5 showed varying de?grees of inhibitory effects on tumor cells in vitro.
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To study the lead excretion effect of the chelator Zn-DTPA on the lead intoxication mice, inductively coupled plasma mass spectrometry (ICP-MS) was applied to detect the lead content of biological samples. The acute lead intoxication mice model was established by injecting lead acetate intraperitoneally with the dose of 1 mg. Zn-DTPA was administered intraperitoneally to mice once daily for five consecutive days 4 h after intoxication. Control group, model group, combination of Zn-DTPA and Ca-DTPA group were evaluated at the same time. The urine was collected every day. The mice were sacrificed in batches in the 2rd, 4th, 6th day. Biological samples including urine, whole blood, femur and brain were prepared and nitrated. Lead concentration was detected by ICP-MS. The result showed that Zn-DTPA could increase lead content in urine markedly and reduce lead content in blood, femur and brain.
Subject(s)
Animals , Mice , Chelating Agents , Pharmacology , Lead , Pharmacokinetics , Urine , Lead Poisoning , Drug Therapy , Mass Spectrometry , Pentetic Acid , PharmacologyABSTRACT
A novel lactone, tripterospermumcins E (1), along with four known compounds, sweroside (2), loganic acid (3), 8-epi-kingiside (4) and bergenin (5), were isolated from the aerial parts of Tripterospermum chinense. Their structures were determined by spectroscopic methods, including 1D and 2D NMR, and chemical methods. Compound 1 is rare beta-lactone with a glucoside.
Subject(s)
Benzopyrans , Chemistry , Drugs, Chinese Herbal , Chemistry , Gentianaceae , Chemistry , Iridoid Glucosides , Chemistry , Iridoids , Chemistry , Lactones , Chemistry , Molecular Structure , Plant Components, Aerial , Chemistry , Plants, Medicinal , ChemistryABSTRACT
In order to find the anti-virus constituents of Alternanthera philoxeroides (Mart.) Griseb, the investigation was carried out. The paper reported the five triterpenoid saponins isolated from n-BuOH fraction: 3-O-beta-D-glucopyranosyl (1-->3)-O-[beta-D-glucopyranosyl-oleanolic acid]-28-O-beta-D-glucuronopyranoside (1), oleanolic acid-3-O-beta-D-glucuronopyranoside (calenduloside E, 2), oleanolic acid-3-O-beta-D-glucopyranosyl-28-Obeta-D-glucopyranosyl ester (chikusetsusaponin-IVa, 3), 3-O-(6'-O-butyl-beta-D-glucuronopyranosyl)-oleanolic acid-28-O-beta-D-glucopyranosyl ester (4) and hederagenin-3-O-beta-D-glucuronopyranoside (HN-sapoins K, 5). 1 is a new compound, saponins 4 and 5 were isolated from the plant for the first time.
Subject(s)
Humans , Amaranthaceae , Chemistry , Antiviral Agents , Chemistry , Pharmacology , Hep G2 Cells , Hepatitis B virus , Molecular Structure , Oleanolic Acid , Chemistry , Pharmacology , Plants, Medicinal , Chemistry , Saponins , Chemistry , Pharmacology , Triterpenes , Chemistry , PharmacologyABSTRACT
To investigate chemical constituents from Radix Pittospori, chloroform extract of the roots was subjected to column chromatography with various chromatographic techniques. The structures were elucidated on the basis of physico-chemical property and spectral analysis. Two triterpenoids were identified as 22-acetyl-21-(2-acetoxy-2-methylbutanoyl)-R1-barrigenol(1) and 3alpha-hydroxyl-20-demethylisoaleuritolic-14(15)-ene-28, 30-dioic acid (2). Compound 1 is a new triterpene and compound 2 is isolated from this plant for the first time.
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Molecular Structure , Plant Roots , Chemistry , Plants, Medicinal , Chemistry , Rosales , Chemistry , Triterpenes , ChemistryABSTRACT
Objective To investigate the chemical constituents of the 60% alcohol extract of Solanum lyratum Thunb..Method The compounds were isolated by column chromatography over silica gel and Sephadex LH-20 and preparative TLC.Their structures were elucidated on the basis of physicochemical property and spectral data.Resulut Eleven compounds were isolated and identified as:ononin(1), genistin(2), 5-hydroxyl ononin(3), formononetin(4), daidzein(5), daidzin(6), 4-hydroxy-benzaldehyde(7),vanillic acid(8), protocatechuic acid(9),ethyl-α-D-arabinofuranoside(10) and ursolic acid(11).Conclusion Compounds 1,2,3,10 and 11 are isolated from S.lyratum for the first time.
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Objective To investigate the chemical constituents of Rannunculus chinensis Bunge..Methods The chemical constituents of R.chinensis were isolated by chromatography on silica gel and Sephadex LH-20. The structures of compounds were identified by phytochemical properties and spectral analysis(MS and NMR).Results Ten compounds were isolated and identified as quercetin(1),kaempferol(2),luteolin(3),quercitrin(4), protocatechuic acid(5),gallic acid(6),ellagic acid(7),kaempferol-3-O-β-rutinoside (8),β-sitosterol (9) and 7-ketologanin (10). Conclusion Compound 10 is isolated from R.chinensis for the first time. Compounds 1,2,3,4,5,6,7 and 8 are obtained from the title plant for the first time.
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The investigation on the herbal of Euphorbia humifusa Wild. was carried out in order to find its anti-HBV constituents. The isolation and purification were performed by chromatography such as Sephadex LH-20, MCI GEL CHP 20P, etc. Based on the spectral analysis, five apigenin glycosides were identified as apigenin-7-O-(6"-O-galloyl)-beta-D-glucopyranoside (1), apigenin-7-O-beta-D-apiofuranosyl (1-->2)-beta-D-glucopyranoside (2), apigenin-7-O-beta-D-lutinoside (3), apigenin-7-O-beta-D-glucopyranside (4) and apigenin (5). Among them, compound 1 is a new compound, compound 2 and 3 were isolated from this plant for the first time.
Subject(s)
Humans , Antiviral Agents , Chemistry , Pharmacology , Apigenin , Chemistry , Pharmacology , Cell Survival , Euphorbia , Chemistry , Glucosides , Chemistry , Pharmacology , Glycosides , Hep G2 Cells , Hepatitis B Surface Antigens , Metabolism , Hepatitis B e Antigens , Metabolism , Molecular Structure , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituent bud of the flowers of Jasminum officinale var. grandiflorum.</p><p><b>METHOD</b>The compounds were isolated and purified by recrystallization and chromatography on silica gel and Sephadex LH - 20 column. Their structures were elucidated on the basis of physicochemical properties and spectral analysis.</p><p><b>RESULT</b>Six triterpenoid saponins were identified as 3-O-alpha-L-rhamnopyranosyl (1 --> 2)-beta-D-xylopyranosyl- hederagenin-28-O-beta-D-galactopyranosyl (1 --> 6)-beta-D-galactopyranosyl ester (1), hederagenin-3-O-beta-D-glucopyranosyl (1 --> 3)-alpha-L-arabinopyranoside (2), 2alpha, 3beta, 23-trihydroxyolean-12-en-28-oic-O-beta-D-glucopyranosyl ester (3), hederagenin-3-O-beta-D-xylopyranosyl (1 --> 3)-alpha-L-rhamnopyranosyl (1 --> 2)-alpha-L-arabinopyranoside (4), 2alpha, 3beta, 23-trihydroxyolean-12-en-28-oic-O-alpha-L-rhamnopyranosyl (1 --> 4)-beta-D-glucopyranosyl (1 --> 6)-beta-D-glucopyranosyl ester (5), hederagenin-3-O-alpha-L-rhamnopyranosyl (1 --> 2)-alpha-L-arabinopyranoside (6).</p><p><b>CONCLUSION</b>Compound 1 is a new compound. Compounds 2, 3, 4, 5, 6 were isolated from the genus Jasminum for the first time.</p>
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Chromatography, Gel , Drugs, Chinese Herbal , Chemistry , Flowers , Chemistry , Jasminum , Chemistry , Magnetic Resonance Spectroscopy , Molecular Structure , Saponins , Chemistry , Triterpenes , ChemistryABSTRACT
To study the chemical constituents of the flowers of Jasminum officinale L. var. grandiflorum, the compounds were isolated and purified by HPLC, recrystallization and chromatography on silica gel and Sephadex LH-20 column. Their structures were elucidated on the basis of physicochemical properties and spectral analysis. Six secoiridoids were identified as jasgranoside (I), jaspolyoside (II), 8-epi-kingiside (III), 10-hydroxy-oleuropein (IV), 10-hydroxy-ligstroside (V), oleoside-7, 11-dimethyl ester (VI). Compound I is a new compound. Compounds II, III, IV, V and VI were isolated from Jasminum officinale L. var. grandiflorum for the first time.
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Flowers , Chemistry , Glucosides , Chemistry , Guaiacol , Chemistry , Iridoid Glucosides , Iridoids , Chemistry , Jasminum , Chemistry , Molecular Structure , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of Peperomia dindygulensis.</p><p><b>METHOD</b>Several column chromatographic methods were used to isolated compounds from P. dindygulensis and spectroscopic methods (1H-NMR, 13C-NMR, HMQC, HMBC, 1D- HOHAHA, NOE) were used to identify the structures of isolated compounds.</p><p><b>RESULT</b>Compound 1 was isolated and identified as 2"-O-beta-D-galactosylisoswertisin.</p><p><b>CONCLUSION</b>Compound 1 was a new compound.</p>
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Chromatography, Affinity , Drugs, Chinese Herbal , Chemistry , Flavones , Galactosides , Magnetic Resonance Spectroscopy , Peperomia , Chemistry , PorosityABSTRACT
To study the chemical constituents of the flower of Jasminum officinale L. var. grandiflorum. The compounds were isolated and purified by re-crystallization and chromatography on silica gel and Sephadex LH-20 column. Their structures were elucidated on the physicochemical properties and spectral analysis. Seven glycosides were identified as kaempferol-3-O-alpha-L-rhamnopyranosyl (1-->3)-[alpha-L-rhamnopyranosyl (1-->6)]-beta-D-galactopyranoside (I), kaempferol-3-O-rutinoside (II), 7-ketologanin (III), oleoside-11-methyl ester (IV), 7-glucosyl-l1-methyl oleoside (V), ligstroside (VI), oleuropein (VII). Compound I is a new compound. Compounds III and V were isolated from the family of Jasminum for the first time and compounds II, IV and VI were isolated from Jasminum officinale L. var. grandiflorum for the first time.
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Flowers , Chemistry , Glucosides , Chemistry , Iridoids , Jasminum , Chemistry , Kaempferols , Chemistry , Oligosaccharides , Chemistry , Plants, Medicinal , Chemistry , Pyrans , ChemistryABSTRACT
To study the chemical constituents of Peperomia dindygulensis Miq. , chromatography was used to isolate and purify the chemical constituents, their structures were elucidated by spectroscopic data and physical-chemical properties. Three compounds were obtained. They were identified as: hypolacetin-4',7-dimethyl ether-8-O-beta-D-glucopyranoside (I) , isoscutellarein-4'-methyl ether-8-O-alpha-L-arabinopyrano-syl-(1--> 4)-beta-D-glucopyranoside ( II) , isoscutellarein-4', 7-dimethyl ether-8-O-alpha-L-arabinopyranosyl-(1-->4) -betea-D-glucopyranoside (III). Compounds I, II and III are all new compounds.
Subject(s)
Disaccharides , Chemistry , Flavones , Chemistry , Flavonoids , Chemistry , Glucosides , Chemistry , Glycosides , Chemistry , Molecular Structure , Peperomia , Chemistry , Plants, Medicinal , ChemistryABSTRACT
<p><b>AIM</b>To study the chemical constituents of Sargentodoxa cuneata.</p><p><b>METHODS</b>To isolate compounds with chromatography technology and to elucidate their structures by spectral analysis.</p><p><b>RESULTS</b>Ten phenolics were isolated from Sargentodxa cuneata and their structures were determined as 1-O-(vanillic acid )-6-O-( 3", 5"-dimethoxy-galloyl)-beta-D-glycoside (I), (-)-epicatechin (II), phydroxyphenylethanol ferulate (III), chlorogenic acid (IV), methyl chlorogenate (V), apocynin (VI), vanillic acid (VII), protocatechuic acid (VIII), 3,4-dihydroxy-phenylethanol (IX), tyrosol (X).</p><p><b>CONCLUSION</b>Compound I is new compound, compound III-VI and VIII-X were isolated from Sargentodoxa cuneata for the first time.</p>